Phytosanitary security is crucial for ensuring the food and national security of Kazakhstan, especially in combating high-risk cereal diseases like leaf rust and stem rust. This study assessed the virulence and race composition of Puccinia triticina and Puccinia graminis populations collected from different regions of Kazakhstan. A total of 30 isolates of leaf and 31 isolates of stem rust were analyzed against Lr- and Sr- near-isogenic lines to determine the effectiveness of resistance genes. Results revealed significant variability in race composition and virulence between northern and southern populations, with some races identified as common to both regions.

The preservation of strain viability and maintenance of their valuable properties are critical prerequisites for all stages of microorganism research, ranging from initial characterization to their application in the production of various biopreparations. This study investigates the revitalization of the virulent Newcastle disease virus (NDV) strain "T-53" in developing chicken embryos (DCE) and evaluates its pathogenic activity in chicks.
After nine years of storage, the biological activity of the "T-53" NDV strain declined to 1.74 lg EID50/ml. Following three successive passages in DCE, the biological activity of the strain was restored to a level suitable for long-term storage. The virus-containing suspension produced was subsequently lyophilized with the addition of a protective medium in accordance with the strain's passport specifications. The quality of the lyophilized strain was evaluated using several key parameters, including appearance, absence of foreign impurities, mold, and ampoule cracks, presence of vacuum, solubility, pH value, moisture content, sterility against bacterial, fungal, and mycoplasmal contaminants, biological activity, and pathogenicity.
The findings confirmed that the physicochemical and biological properties of the revitalized "T- 53" NDV strain correspond to the specifications outlined in its passport, ensuring its suitability for further use and long-term preservation.

The article presents the results of a study on the safety and immunogenicity of monoand polyvalent vaccines designed for the specific prevention of pasteurellosis in animals, based on the genetically attenuated strain of P. multocida Aro/A serotype B.
The monovalent vaccine, made from P. multocida serotype B, was administered to calves in a dose of 2.5x108 CFU (colony-forming units), and to sheep and goats in a dose of 2.5x109 CFU. The polyvalent vaccine, containing a genetically attenuated strain of P. multocida Aro/A and microbial cells of P. multocida serotype D in equal proportions (1:1), was administered to calves and piglets in a dose of 2.5x108 CFU, and to sheep and goats in a dose of 2.5x109 CFU.
To evaluate the immunogenicity of the vaccines, animals were infected with the epizootic strain of P. multocida T88 on the 28th day after vaccination. Following infection, no signs of pasteurellosis were observed in any of the experimental animals: there were no indications of pain, redness or rashes at the body and injection sites, weakness, coughing, or elevated body temperature.
The results of the conducted studies confirm the safety and immunogenicity of the mono- and polyvalent vaccines prepared from the attenuated strain of P. multocida Aro/A for animals.

This paper discusses the principles and methodology of validation of an analytical method based on high performance liquid chromatography (HPLC) used for quality control and quantitative determination of the active substance in an anthelmintic drug.
Key validation parameters such as specificity, linearity, accuracy, precision, detection limit, and quantification limit are described. The analysis made it possible to confirm the compliance of the method with the requirements of regulatory documentation, which ensures its suitability for subsequent quality control of the drug. The results obtained indicate the high reliability and reproducibility of the HPLC method in the conditions of laboratory control of pharmaceutical products. It has been established that for all the parameters presented, the method meets the requirements of regulatory documents.

Newcastle disease represents one of the key transboundary infections of importance for the veterinary service of Kyrgyzstan. According to the Ministry of Agriculture of the Kyrgyz Republic and international organisations (OIE/WOAH), the disease has been reported in different regions of the country, especially in the southern regions where poultry farming is common among private households.
Newcastle disease is widespread on all continents, except Australia, and has a significant negative impact on the development of the poultry industry, causing major economic losses. In the Kyrgyz Republic, cases of the disease were recorded in 2015 and 2016, among others. Despite the fact that the causative agent of the disease is well studied and the clinical course of the infection is known, the issue of complete elimination of the disease remains unresolved. The aim of this review article is to systematise and summarise the available literature, including historical data, epizootological data, molecular and genetic characteristics of the pathogen, as well as modern approaches to the diagnosis, prevention and control of Newcastle disease. Special attention is paid to the epizootic situation in the Kyrgyz Republic.

This review article discusses the methods and technologies of vaccine lyophilization. It analyzes the main stages of the process as well as certain approaches to optimizing sublimation conditions to improve the efficiency and quality of the final product. Special attention is paid to the role of protective media in ensuring the stability, safety, and biological activity of vaccines, as well as in expanding their storage and transportation conditions. The presented review provides a comprehensive overview of the current state of lyophilization technologies in vaccine production and identifies promising directions for future research in this field.