The work contains the results of quality control of an experimental series of inactivated associated vaccine against Newcastle disease (ND) and avian influenza (AI) produced at the Research Institute for Biological Safety Problems of the Ministry of Health of the Republic of Kazakhstan. The purpose of the study was to test to determine the main indicators - the physicochemical properties of an experimental series of inactivated associated vaccine against BN and AI. The results of the experimental series showed that the vaccine is without contaminants, the concentration of hydrogen ions (pH) is 7.29 ± 0.0002137, the kinematic viscosity of the emulsion is 35.37 ± 0.00944, the vaccine is immunogenic for 12 month old chickens and harmless for chickens 30 days old . The average titers of antibodies to the virus on the 28th day after vaccination averaged 1:24.5 against the HP virus and 1:27.3 against the ND virus 1:24.5 in the hemagglutination inhibition test (HAI). The vaccine meets all the requirements in its physical and biological properties and is suitable for an associated vaccine against ND and AI.

The vaccine meets all quality requirements according to the scientific and technical documentation of the manufacturer.

The emergence of new variants of the SARS-CoV-2 coronavirus is caused by mutations in the main structural proteins of severe acute respiratory syndrome coronavirus 2. Vaccination and other therapeutic approaches can help stop the epidemic. Scientists are currently developing drugs and vaccines that specifically target the structural proteins of the SARS-CoV-2 coronavirus. As a result, taking into account mutations in proteins and determining their impact on function will help in high-quality production and development of preventive and therapeutic agents. As a result of sequencing, it was possible to obtain the complete nucleotide sequence of the E gene of the SARS-CoV-2 coronavirus. The presence and location of mutations in the envelope protein (E) of SARS-CoV-2 virus isolates were investigated by aligning the sequences with the reference sequence of the SARS-CoV-2 virus. The results showed that the most relative mutations in the amino acid sequence of the E protein of the SARS-CoV-2 coronavirus occurred in regions 9 and 11. Two mutations, T9I and T11A, were found compared with the Severe acute respiratory syndrome coronavirus 2 (NC 045512.2) strain. The identified structural mutations of the E protein can be used in the strategy for developing drugs and vaccines.

The paper presents the results of determining the optimal immunising dose of inactivated vaccine against camelpox from the strain "KM - 40", obtained from the Republican Depository of especially dangerous pathogens of the Research Institute of Biological Safety Problems. The results of studies showed that intramuscular administration of inactivated vaccine against camelpox on camel rats formed antibodies to camelpox virus, the titre of which depended on the dose of the preparation. At 14 days after vaccination no antibodies were detected in the blood serum of camels immunised with the vaccine in the volumes of 2.0 and 3.0 ml, whereas in animals immunised in the volume of 5.0 ml the antibody titre was 1:2 and 1:4 in the neutralisation reaction and diffusion precipitation reaction, respectively. For use in the field, we recommend the use of 5.0 ml vaccine. If all the requirements specified in the regulations on vaccine production and control are met, this dose of the vaccine preparation ensures the formation of intense immunity against camelpox.

The article presents the results of studies to determine the immunogenicity of sheep pox vaccine based on the G20-LKV strain of goat pox virus. In experiments conducted on sheep aged 6-12 months, it was found that a heterogeneous sheep pox vaccine has high immunogenicity for sheep.Immunization caused the formation of viral neutralizing antibodies in the blood of sheep in high titers and ensured the formation of intense immunity, which was confirmed by the results of control infection of sheep with virulent sheep pox virus, strain "Afghan". The animals of the control group reacted to infection and became ill with characteristic clinical signs of sheep pox (hyperthermia, papules and pustules at the injection site).Vaccinated animals did not have clinical signs characteristic of sheep pox, during 14 days of observation. The general condition of the animals was satisfactory, no side effects, swelling and development of the smallpox process at the injection site were detected.

This review analyzes the qualitative and quantitative content of biologically active substances in the raw material of camel thorn (Alhage pseudalhagi). During the study, the extract from the plant material Alhage pseudalhagi was obtained with the help of ultrasound. The method of determining the amount of flavonoids, antioxidants and other important biologically active compounds in plant raw materials with biological properties is considered. It has been established that the ultrasonic extraction method allows obtaining a large amount of flavonoids and extractive substances. The technology of ultrasonic extraction is developed and the obtained extract is identified by the method of high performance liquid chromatography. The obtained results give information about the high possibilities of ultrasonic processing to increase the output of biologically active compounds from raw materials.

In recent years, digital innovations have become widespread in the agro-industrial complex and in the agricultural business. The conversion of veterinary documentation, the system of animal registration and food safety control into digital format is an urgent task for the veterinary medicine of Kazakhstan. The introduction of digital technologies is necessary to process huge amounts of valuable information that remain unused in the control of the epizootic situation and food safety. Veterinary documentation is still kept on paper, which takes a lot of time. Manual entry of primary information creates numerous errors and inaccuracies and negatively affects the completeness of the information, making it impossible to keep operational statistics when monitoring the epizootic situation.

The purpose of this work is to develop a digital platform with a mobile application that allows you to automate the processes of generating and transferring information about animals to the ILI database (Identification of Farm Animals), free practical veterinary workers from the shaft of paperwork, and raise work to control the epizootic situation to a qualitatively new level. and safety of livestock products, promptly monitor the movement of livestock across the country, especially from the vaccination zone to clean zones.

The materials of the study are legal documentation, statistical indicators of animal husbandry in the Akkol district of the Akmola region, operational veterinary reporting. The research methods were based on the generalization of information obtained as a result of a comparative analysis of the time spent using the developed mobile application and the current system for identifying farm animals during veterinary activities.

A digital platform has been developed - a mobile application "Mal Derek", which ensures the quality and reliability of primary material entered into the veterinary database automatically and in digital format. The author's certificate for this computer program has been received. Work was carried out to test the functionality of the mobile application "Mal Derek" in the Akkol district of the Akmola region with the participation of veterinary specialists. Automating the process of transferring information about animals from local veterinary specialists to veterinary laboratories, to the laboratory of veterinary and sanitary examination of markets and trade organizations, to enterprises for the processing of animal products, as well as to the central database, opens up new possibilities for solving the problem of ensuring the safety of animal products.