REAL-TIME POLYMERASE CHAIN REACTION USING SYBR GREEN I FLUORESCENT DYE TO DIAGNOSE CANINE DISTEMPER VIRUS

The canine distemper virus was diagnosed using real-time polymerase chain reaction (RT-PCR). For diagnostics with RT-PCR, the fluorescent dye SYBR Green I was used. Using RT-PCR, 106 dogs were tested in the vicinity of Bishkek and c. Bishkek. Of 106 dogs, 10 healthy dogs were used for controls. Clinical samples were obtained from 96 dogs, from 87 the causative agent of canine distemper virus was identified and 9 gave a negative result. In recent years, the PCR method has been successfully used in veterinary practice and in scientific centers of the our country to diagnose infectious diseases of agricultural and domestic animals. In veterinary practice and science, when determining infectious diseases, PCR is a standard procedure that allows you to determine the presence of the pathogen gene. With canine distemper virus, the use of the PCR method is considered a necessary procedure, since other methods are less sensitive and not very effective. The use of RT-PCR simplifies diagnostics and saves time for the analysis than PCR in the classic version. Therefore, today, the introduction of RT-PCR into practical veterinary medicine is considered relevant in our country.

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