one of the most important issues in veterinary medicine and agriculture is the preservation and reproduction of livestock. During the Soviet Union era, camel breeding developed only in the western regions of Kazakhstan, and in the post-independence years it developed rapidly in the southern and south-eastern regions of the country. However, in recent years, the registration of camelpox has become a new problem in veterinary medicine, creating some obstacles to the development of camel breeding. This is evidenced by the outbreak of camelpox in the Mangistau region in 2019, which led to a decrease in livestock productivity and a decrease in livestock.

In order to learn more about this infectious disease, which is considered the most dangerous for camels, the work of domestic and foreign scientists was conducted. The search was conducted using keywords in English and Russian from Internet resources. As a result of the research, 48 scientific works were analyzed, including 44 foreign articles published in English, 2 works in Russian, 2 research works of domestic scientists. This scientific review article provides a review of the scientific literature on the general description, distribution, epizootology, diagnosis and prevention of camelpox. The article summarizes and presents all the information on these sections in the form of an abstract in the state language.

dourine is diagnosed using serological tests such as the classical complement fixation test (CFT), the horse complement fixation test (HCFT). We have recently developed a long-term variant of HCFT - horse complement long fixation (HCLFT). The main component that directly affects the sensitivity of the above tests is the trypanosomal antigen. From the correct definition of the working titer of the latter depends on the clarity of setting and the course of reactions. The aim of our work was to determine the working titer of the trypanosomal antigen in CFT, HCFT and HCLFT. The working antigen titer for each serological reaction remains stable after its determination. This indicator was determined by the checkerboard method of titration of the antigen and positive serum. We have established the working titer of the antigen in the antigenic unit (AU). At the same time, we took 1,5 AU for the working titer of the trypanosome antigen. It was 1:20 in CFT, 1:100 in HCFT and 1:150 in HCLFT. It is these dilutions of the antigen that we will use when setting up the above tests.

as you know, one of the economically and socially significant diseases that are widespread in the territory of the Republic of Kazakhstan is brucellosis, which occupies a dominant place in the general infectious pathology of animals. Brucellosis is one of the most dangerous zoonotic infectious diseases for humans. The results of comparative studies on the identification of the causative agent of brucellosis in the Republic of Kazakhstan for several years using the bacteriological method and PCR indicate that the use of the above methods to determine the epizootological status of herds of animals in the primary diagnosis of brucellosis is impractical due to the low degree of informativeness of these tests. PCR is recommended for identification and genotyping of isolated brucella cultures from pathological material.

         As a result of diagnostic studies of biomaterial obtained from animals from economically disadvantaged brucellosis subjects of Kazakhstan and border countries (Russia and Kyrgyzstan), the authors identified brucella cultures, which were subsequently subjected to the study of their biological and molecular genetic properties. For the identification and genotyping of isolated brucella cultures, researchers suggest using MLVA as the most effective method for reliably determining their genotypic characteristics.

the paper presents the results of an approbation test of the physical and biological properties of an experimental series of a vaccine against Newcastle disease from the La-Sota strain in the conditions of the National Reference Center for Veterinary Medicine of the Committee for Veterinary Control and Supervision of the Ministry of Agriculture of the Republic of Kazakhstan (CVC and SMA RK) and the Research Institute of Biological Problems security (RIBSP). The aim of the study was to test a vaccine against Newcastle disease from the La-Sota strain. When performing the work, general virological and immunological research methods were used. The results of approbation studies showed that the vaccine was free of contaminants and its biological activity was 9,03 ± 0,14 lg EID₅₀/cm³. The vaccine is harmless to chickens of 40 days of age. The vaccine is immunogenic for chickens. The average titers of antibodies to the Newcastle disease virus on the 16th day after vaccination is 1:127,2 in the hemagglutination inhibition test (HITA). The vaccine meets all the required requirements in terms of its physico-biological properties and is suitable for specific prophylaxis of Newcastle disease. Approbation tests of the physical, biological properties of the vaccine were completed with positive results and protocols, research acts were signed by members of the Commission of the CVC and SMA RK. It is recommended to use the vaccine on a large scale in the conditions of poultry farms, private farmsteads for the specific prevention of Newcastle disease.

vaccine production is considered the most effective way to prevent and control the spread of infectious diseases. The transplantable Vero cell line is now widely used for vaccine production.

The aim of this study was to investigate the optimal parameters for growing Vero cell cultures in cell factories for large-scale vaccine production. For the scaling process, the seed concentration of cells was determined. The results of the studies showed that at a cell inoculum concentration of 2.0x105 cells/ml, a monolayer is formed in 1-2 days and is the most optimal for cultivating most viruses. Multilayer cell culture systems for industrial scales provide a one-stop production solution. The convenient and cost-effective format of cell factories saves space, time and labor and reduces the risk of contamination.

In this paper, Vero cell culture is scaled up in cell factories because this cell culture method is necessary to create cost-effective workflows in large volumes. The study showed that the inoculum concentration, the cell proliferation index, and the ratio of the dispersing mixture for cell removal are comparable to routinely used T-vials.

domestic dogs and cats play an important role in the transmission of the helminthic zoonotic agent, like the toxocara worm, which is directly transmitted from domestic animals to the human environment without the involvement of vectors or intermediate hosts. Toxocariasis is a parasitic disease caused by the dog and cat roundworms Toxocara canis and Toxocara cati, respectively. The prevalence of toxocariasis among the population of Tajikistan remains unknown. The number of the main owners of toxocariasis - dogs and cats in the Republic of Tajikistan from 2017 to 2021 is approximately 7058 and 1262, respectively, and tends to increase. The high density of free-roaming dogs and cats maintains a constant infection pressure of these and other parasites. Continuing education of veterinarians and informing pet owners through the provision of unified advice is a priority. Close collaboration between veterinarians and public health professionals is required within the One Health concept.

in the process of pathoanatomical autopsy of dead and sick rabbits from an unknown disease, biomaterials were selected in order to determine the cause of the disease and death of rabbits.

Biomaterials were examined for the presence of pathogens of pasteurellosis, clastridium, viral hemorrhagic disease of rabbits.

In the experiment, polymerase chain reaction and complement fixation reaction were used, it was experimentally established that the cause of the disease and death of rabbits is the virus Rabbit Hemorrhagic Disease.