TREPONEMA PALLIDUM-ДЫ IN VITRO КУЛЬТИВАЦИЯЛАУ: ҚОЯН МОДЕЛІНЕН АДАМ ЖАСУШАЛЫҚ ЖҮЙЕЛЕРІНЕ ДЕЙІН

Treponema pallidum subsp. pallidum - мерездің қоздырғышы болып табылатын, адам денсаулығы үшін әлеуметтік маңызы жоғары инфекциялық агент. Тиімді бактерияға қарсы емдеу әдістерінің болуына қарамастан, бұл патогеннің биологиясын зерттеу ұзақ уақыт бойы оны жасанды жағдайда тұрақты культивациялау мүмкіндігінің шектеулі болуына байланысты күрделі мәселе болып келді. Шолудың мақсаты - T. pallidum-ды in vitro культивациялау жүйелерінің дамуы туралы қазіргі деректерді жүйелеу: қоян моделінен және ерте тіндік культуралардан бастап Sf1Ep платформасына, заманауи клиникалық штаммдарды тікелей бөліп алуға және адам жасушаларына негізделген жүйелердің пайда болуына дейін. Шолуда T. pallidum-ды культивациялауды қиындататын биологиялық факторлар, соның ішінде геномның редукциясы, метаболизмдік мүмкіндіктердің шектеулілігі, иесі жасушасына тәуелділік және оттегіге сезімталдық қарастырылды. T. pallidum-ды in vitro культивациялау қоздырғыштың физиологиясын, иесі жасушаларымен өзара әрекеттесуін, антибиотиктерге сезімталдығын зерттеуге, жануарлар модельдеріне тәуелділікті азайтуға және биоқауіпсіз эксперименттік тәсілдерді дамытуға арналған перспективалы ғылыми-технологиялық платформа ретінде қарастырылуы тиіс.

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