RESTORATION OF VIRULENCE OF B.MELITENSIS 16 M STRAINS AND BRUCELLA ABORTUS 544
N. N. Zinina,
B. A. Yespembetov,
N. Syrym,
S. E. Alpysbaeva,
M. K. Sarmykova,
A. R. Abdymukhtar,
Y. B. Serikbay,
A. T. Toleukhan,
A. M. Anarbekova,
M. M. Maulenbayeva https://doi.org/10.58318/2957-5702-2023-14-35-40
Abstract
When testing anti-brucellosis vaccines, an important role is played by studying the infecting dose of control strains. To restore the virulent properties of the control strains Brucella melitensis 16 M and Brucella abortus 544, it is necessary to carry out multiple successive passages in different doses (starting from 1,000,000 colony-forming units, CFU/cm3 to 5 CFU/cm3), through the body of sensitive laboratory animals. In this case, for experimental work, we took 54 heads of guinea pigs (males) weighing 350-380 g, from which we created 9 groups of 6 heads each. The article reflects the progress and results of research into determining the minimum fixing infectious dose of the control reference strains Brucella melitensis 16 M and Brucella abortus 544.
Based on the results of the studies, the minimum infectious dose (5 CFU) of Brucella after the ninth passage, which causes the generalized form of brucellosis, was determined. Spent virulent fixing doses (5 CFU/cm3) strains of Brucella melitensis 16 M and Brucella abortus 544 can be used for control infection when setting up various experiments to determine and evaluate the immunogenicity of anti-brucellosis vaccines and chemoprophylaxis.
Supporting agencies: LPP Research institute for biological safety problems
About the Authors
N. N. Zinina
«Research Institute for Biological Safety Problems» Ministry of Health of the Republic of Kazakhstan
Kazakhstan
Kazakhstan
Leading Researcher
Guardeysky
B. A. Yespembetov
«Research Institute for Biological Safety Problems» Ministry of Health of the Republic of Kazakhstan
Kazakhstan
Kazakhstan
Head of Laboratory
Guardeysky
N. Syrym
«Research Institute for Biological Safety Problems» Ministry of Health of the Republic of Kazakhstan
Kazakhstan
Kazakhstan
Chief Researcher
Guardeysky
S. E. Alpysbaeva
«Research Institute for Biological Safety Problems» Ministry of Health of the Republic of Kazakhstan
Kazakhstan
Kazakhstan
Senior Researcher
Guardeysky
M. K. Sarmykova
«Research Institute for Biological Safety Problems» Ministry of Health of the Republic of Kazakhstan
Kazakhstan
Kazakhstan
Researcher
Guardeysky
A. R. Abdymukhtar
«Research Institute for Biological Safety Problems» Ministry of Health of the Republic of Kazakhstan
Kazakhstan
Kazakhstan
Researcher
Guardeysky
Y. B. Serikbay
«Research Institute for Biological Safety Problems» Ministry of Health of the Republic of Kazakhstan
Kazakhstan
Kazakhstan
Senior laboratory assistant
Guardeysky
A. T. Toleukhan
«Research Institute for Biological Safety Problems» Ministry of Health of the Republic of Kazakhstan
Kazakhstan
Kazakhstan
Junior Researcher
Guardeysky
A. M. Anarbekova
«Research Institute for Biological Safety Problems» Ministry of Health of the Republic of Kazakhstan
Kazakhstan
Kazakhstan
Junior Researcher
Guardeysky
M. M. Maulenbayeva
«Research Institute for Biological Safety Problems» Ministry of Health of the Republic of Kazakhstan
Kazakhstan
Kazakhstan
Laboratory assistant
Guardeysky
References
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4. Manual of diagnostic tests and vaccines for terrestrial animals (mammals, birds and bees) Sixth Edation Volume 2, 2008. – P.38.
Review
For citations:
Zinina N.N., Yespembetov B.A., Syrym N., Alpysbaeva S.E., Sarmykova M.K., Abdymukhtar A.R., Serikbay Y.B., Toleukhan A.T., Anarbekova A.M., Maulenbayeva M.M. RESTORATION OF VIRULENCE OF B.MELITENSIS 16 M STRAINS AND BRUCELLA ABORTUS 544. Biosafety and Biotechnology. 2023;(14):35-40. (In Russ.) https://doi.org/10.58318/2957-5702-2023-14-35-40
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