biobБиобезопасность и БиотехнологияBiosafety and Biotechnology2707-72412957-5702Научно-исследовательский институт проблем биологической безопасности10.58318/2957-5702-2023-15-62-71biob-57Research ArticleСТАТЬИArticlesВЫЯВЛЕНИЕ ХЛАМИДИЯ-ПОДОБНОГО МИКРООРГАНИЗМА WADDLIA CHONDROPHILA У КЛЕЩЕЙDETECTION OF CHLAMYDIA-LIKE MICROORGANISM WADDLIA CHONDROPHILA IN TICKShttps://orcid.org/0000-0002-6976-1540ШыныбековаГ. О.ShynybekovaG. O.

Шыныбекова Гаухар Орынбековна, научный сотрудник лаборатории "Молекулярная биология и генная инженерия",

пгт. Гвардейский

Gvardeysky

sh.gaukhar@biosafety.kzhttps://orcid.org/0000-0002-5151-8120МухамиН. Н.MukhamN. N.

Мухами Назым Нурғайқызы, и.о. научного сотрудника лаборатории "Молекулярная биология и генная инженерия"

пгт. Гвардейский

Gvardeysky

n.n.mukhami@biosafety.kzhttps://orcid.org/0000-0002-5623-7571ИсабекА. У.IsabekA. U.

Исабек Айша Уранқызы, и.о. старшего научного сотрудника лаборатории "Молекулярная биология и генная инженерия"

пгт. Гвардейский

Gvardeysky

isabekova___aisha@mail.ruhttps://orcid.org/0000-0001-6299-9399КожабергеновН. С.KozhabergenovN. S.

Кожабергенов Нурлан Сиязбекович, старший научный сотрудник лаборатории «Молекулярная биология и генная инженерия»

пгт. Гвардейский

Gvardeysky

n.kozhabergen@biosafety.kzhttps://orcid.org/0000-0002-7954-6246ЧервяковаО. В.ChervyakovaO. V.

Червякова Ольга Викторовна, главный научный сотрудник лаборатории «Молекулярная биология и генная инженерия»

пгт. Гвардейский

Gvardeysky

o.chervyakova@biosafety.kzhttps://orcid.org/0000-0002-1332-1247СултанкуловаК. Т.SultankulovaK. T.

Султанкулова Куляйсан Турлыбаевна, заведующий лабораторей «Молекулярная биология и генная инженерия»

пгт. Гвардейский

Gvardeysky

k.sultankulova@biosafety.kz«Научно-исследовательский институт проблем биологической безопасности» МЗ РККазахстан"Scientific Research Institute of Biological Safety Problems" of the Ministry of Health of the Republic of KazakhstanKazakhstan2023110920240156271Copyright © Шыныбекова Г.О., Мухами Н.Н., Исабек А.У., Кожабергенов Н.С., Червякова О.В., Султанкулова К.Т., 20242024Шыныбекова Г.О., Мухами Н.Н., Исабек А.У., Кожабергенов Н.С., Червякова О.В., Султанкулова К.Т.Shynybekova G.O., Mukham N.N., Isabek A.U., Kozhabergenov N.S., Chervyakova O.V., Sultankulova K.T.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.biosafety.kz/jour/article/view/57

Хламидия-подобный микроорганизм Waddlia chondrophila (W. Сhondrophila) является новым патогеном, который вызывает выкидыши и аборты животных.

Цель этого исследования состояла в выявлении хламидии у иксодовых клещей, собранных с крупного рогатого скота. Полимеразная цепная реакция (ПЦР) и секвенирование были использованы для подтверждения присутствия возбудителя W. Сhondrophila в образцах клещей, собранных у домашних животных.

Анализ нуклеотидных последовательностей показал, что в образцах клеща Dermacentor marginatus выявлены ДНК бактерии W. chondrophila. Среди 156 образцов клещей, собранных из Северо-Казахстанской, Западно-Казахстанской, Кызылординской, Жамбылской и Туркестанской областей, 7% были положительными на хламидия-подобный микроорганизм W. chondrophila.

Наличие ДНК хламидия-подобного микроорганизма W. chondrophila у иксодовых клещей свидетельствует о необходимости проведения дополнительных исследовании для изучения потенциальной роли клещей как переносчиков данной зоонозной бактерий.

Chlamydia-like microorganism Waddlia chondrophila (C. Chondrophila) is a new pathogen that causes miscarriages and abortions in humans and cattle. This bacterium is considered as a potential zoonotic agent. The main reservoirs and carriers of C. chondrophila are ixodic ticks, and determining their role remains the most difficult and interesting question to be solved in future studies.

The purpose of this study was to identify chlamydia in ixodic ticks collected from cattle by molecular methods. Polymerase chain reaction (PCR) and sequencing were used to confirm the presence of the pathogen in tick samples collected from domestic animals.

The analysis of nucleotide sequences showed that the DNA of the bacterium W. chondrophila was detected in the ticks Dermacentor marginatus, Hyalomma asiaticum, Hyalomma marginatum, Ixodes persulcatus, Hyalomma anatolicum. Among 156 tick samples collected from North Kazakhstan, West Kazakhstan, Zhambyl and Turkestan regions, 7% were positive for chlamydia-like microorganism W. chondrophila.

The presence of DNA of the chlamydia-like W. chondrophila microorganism in ixodic ticks suggests that additional research is needed to study the potential role of ticks as carriers of these zoonotic bacteria.

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The authors declare that there are no conflicts of interest present.