biobБиобезопасность и БиотехнологияBiosafety and Biotechnology2707-72412957-5702Научно-исследовательский институт проблем биологической безопасностиbiob-183Research ArticleМЕДИЦИНСКАЯ И ВЕТЕРИНАРНАЯ БИОТЕХНОЛОГИЯMEDICAL AND VETERINARY BIOTECHNOLOGYМИКРОНОСИТЕЛИ В СУСПЕНЗИОННОЙ КУЛЬТУРЕ КЛЕТОК VERO: ПОДХОД К ЭФФЕКТИВНОЙ РЕПЛИКАЦИИ И МАСШТАБИРОВАНИЮ ЧМЖЖ (ШТАММ NIGERIA 75/1)https://orcid.org/0000-0002-2332-2841СаметоваЖ. Ж.zh.sametova@biosafety.kzhttps://orcid.org/0000-0002-3987-6814АмановаЖ. Т.zh.amanova@biosafety.kzhttps://orcid.org/0000-0002-9515-0655ТұрыскелдіШ. С.sh.smankizi@biosafety.kzhttps://orcid.org/0000-0003-3639-3793ҮсембайА. Қ.a.ussenbay@biosafety.kzhttps://orcid.org/0000-0001-5609-2491АбитаевР. Т.r.abitaev@biosafety.kzhttps://orcid.org/0000-0002-8224-8047КондибаеваЖ. Б.zh.kondibaeva@biosafety.kzhttps://orcid.org/0000-0001-8543-4219БулатовЕ. А.ye.bulatov@biosafety.kz202512022026123№23 (2025)183Copyright © Саметова Ж.Ж., Аманова Ж.Т., Тұрыскелді Ш.С., Үсембай А.Қ., Абитаев Р.Т., Кондибаева Ж.Б., Булатов Е.А., 20262026Саметова Ж.Ж., Аманова Ж.Т., Тұрыскелді Ш.С., Үсембай А.Қ., Абитаев Р.Т., Кондибаева Ж.Б., Булатов Е.А.Саметова Ж.Ж., Аманова Ж.Т., Тұрыскелді Ш.С., Үсембай А.Қ., Абитаев Р.Т., Кондибаева Ж.Б., Булатов Е.А.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.biosafety.kz/jour/article/view/183

В настоящем исследовании оценивалась репродукция штамма Nigeria 75/1 вируса чумы мелких жвачных животных (ЧМЖЖ) в культуре клеток Vero, культивируемых в суспензионных условиях с использованием микроносителей. Данный подход представляет собой современную платформу, сочетающую преимущества суспензионного культивирования с высокой удельной продуктивностью клеток, обеспечиваемой адгезией на поверхности микроносителей.

Было исследовано влияние различных параметров – типа микроносителей (Cytodex 1 и Cytodex 3), посевной концентрации клеток Vero, инфицирующей дозы вируса и длительности культивирования на продуктивность системы. Экспериментальные результаты показали, что использование микроносителей обеспечивает надежную адгезию и активную пролиферацию клеток Vero, создавая оптимальные условия для репликации вируса. Полученный вирусный титр (≥ 6.0 lg ТЦД50/см³) превышал показатели, описанные при традиционном монослойном культивировании, что свидетельствует о повышенной эффективности репродукции вируса. Таким образом, полученные данные подтверждают высокую перспективность технологии культивирования с применением микроносителей как в экспериментальных исследованиях, так и для промышленной разработке и производства вакцин против ЧМЖЖ.

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The authors declare that there are no conflicts of interest present.