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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">biob</journal-id><journal-title-group><journal-title xml:lang="ru">Биобезопасность и Биотехнология</journal-title><trans-title-group xml:lang="en"><trans-title>Biosafety and Biotechnology</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2707-7241</issn><issn pub-type="epub">2957-5702</issn><publisher><publisher-name>Научно-исследовательский институт проблем биологической безопасности</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.58318/2957-5702-2022-9-44-52</article-id><article-id custom-type="elpub" pub-id-type="custom">biob-17</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>БИОЛОГИЧЕСКАЯ БЕЗОПАСНОСТЬ И БИОЗАЩИТА</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>BIOLOGICAL SAFETY AND BIOSECURITY</subject></subj-group></article-categories><title-group><article-title>МАСШТАБИРОВАНИЕ КУЛЬТУРЫ КЛЕТОК VERO  ДЛЯ ПРОИЗВОДСТВА БИОПРЕПАРАТОВ</article-title><trans-title-group xml:lang="en"><trans-title>SCALING OF VERO CELL CULTURE FOR THE PRODUCTION  OF BIOLOGICAL PRODUCTS</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Сейдахметова</surname><given-names>Б. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Seidakhmetova</surname><given-names>B. A.</given-names></name></name-alternatives><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Жаппарова</surname><given-names>Г. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Zhapparova</surname><given-names>G. A.</given-names></name></name-alternatives><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Мараховская</surname><given-names>Л. Г.</given-names></name><name name-style="western" xml:lang="en"><surname>Marakhovskaya</surname><given-names>L. G.</given-names></name></name-alternatives><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Теребай</surname><given-names>А. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Terebay</surname><given-names>A. A.</given-names></name></name-alternatives><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Наханов</surname><given-names>А. К.</given-names></name><name name-style="western" xml:lang="en"><surname>Nakhanov</surname><given-names>A. K.</given-names></name></name-alternatives><email xlink:type="simple">aziz_nk@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">Научно-Исследовательский институт проблем биологической безопасности<country>Казахстан</country></aff><aff xml:lang="en">Scientific Research Institute of Biological Safety Problems<country>Kazakhstan</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2022</year></pub-date><pub-date pub-type="epub"><day>27</day><month>11</month><year>2022</year></pub-date><volume>0</volume><issue>9</issue><fpage>44</fpage><lpage>52</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Сейдахметова Б.А., Жаппарова Г.А., Мараховская Л.Г., Теребай А.А., Наханов А.К., 2022</copyright-statement><copyright-year>2022</copyright-year><copyright-holder xml:lang="ru">Сейдахметова Б.А., Жаппарова Г.А., Мараховская Л.Г., Теребай А.А., Наханов А.К.</copyright-holder><copyright-holder xml:lang="en">Seidakhmetova B.A., Zhapparova G.A., Marakhovskaya L.G., Terebay A.A., Nakhanov A.K.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://journal.biosafety.kz/jour/article/view/17">https://journal.biosafety.kz/jour/article/view/17</self-uri><abstract><p>производство вакцины считается наиболее эффективным способом предотвращения распространения инфекционных заболеваний и борьбы с ними. В настоящее время перевиваемая клеточная линия Vero широко используется для производства вакцин. Целью настоящего исследования состояла в том, чтобы  изучить оптимальные параметры выращивания культуры клеток Vero на клеточных фабриках для осуществления крупномаштабного производства вакцин. Для процессов масштабирования необходимо было определить посевную концентрацию клеток, сроки образования клеточного монослоя, урожай клеток и параметры диспергирования клеток. Результаты исследований показали, что при посевной концентрации клеток составляющей 2.0х105 кл/мл монослой образуется на 1-2 сут и является наиболее оптимальной для культивирования большинства вирусов. Многослойные системы для культивирования клеток в промышленных масштабах представлят собой универсальное решение для производства. Удобный и выгодный формат клеточных фабрик позволяет экономить пространство, время и трудозатраты и снижает риск контаминации. В данной работе проведены масштабирование культуры клеток Vero на клеточных фабриках, потому что такой метод выращивания клеток является необходимым для создания экономически эффективных рабочих процессов в больших объемах. В проведенном исследовании показано, что посевная концентрация, индекс пролиферации клеток и соотношение диспергирующей смеси для снятия клеток сопоставимы с рутинно используемыми Т-флаконами.</p></abstract><trans-abstract xml:lang="en"><p>vaccine production is considered the most effective way to prevent and control the spread of infectious diseases. The transplantable Vero cell line is now widely used for vaccine production.</p><p>The aim of this study was to investigate the optimal parameters for growing Vero cell cultures in cell factories for large-scale vaccine production. For the scaling process, the seed concentration of cells was determined. The results of the studies showed that at a cell inoculum concentration of 2.0x105 cells/ml, a monolayer is formed in 1-2 days and is the most optimal for cultivating most viruses. Multilayer cell culture systems for industrial scales provide a one-stop production solution. The convenient and cost-effective format of cell factories saves space, time and labor and reduces the risk of contamination.</p><p>In this paper, Vero cell culture is scaled up in cell factories because this cell culture method is necessary to create cost-effective workflows in large volumes. The study showed that the inoculum concentration, the cell proliferation index, and the ratio of the dispersing mixture for cell removal are comparable to routinely used T-vials.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>культура клеток Vero</kwd><kwd>монослой</kwd><kwd>клеточная фабрика</kwd><kwd>вакцина</kwd><kwd>индекс пролиферации</kwd></kwd-group><kwd-group xml:lang="en"><kwd>vero cell culture</kwd><kwd>cell factory</kwd><kwd>vaccine</kwd><kwd>proliferation index</kwd><kwd>DMEM culture medium</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Hu W.S. Overview of cell culture processes // J.Cell Culture Bioprocess Engineering (Boca Raton: CRC Press). – 2020. – P.1–35.</mixed-citation><mixed-citation xml:lang="en">Hu W.S. 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