biobБиобезопасность и БиотехнологияBiosafety and Biotechnology2707-72412957-5702Научно-исследовательский институт проблем биологической безопасности10.58318/2957-5702-2023-16-18-27biob-140Research ArticleСТАТЬИArticlesОПРЕДЕЛЕНИЕ МУТАЦИИ Е БЕЛКА В ИЗОЛЯТАХ ВИРУСА SARS-CОV-2DETERMINATION OF E PROTEIN MUTATION IN SARS-COV-2 VIRUS ISOLATEShttps://orcid.org/0000-0003-2044-7849ЖунушовА. Т.ZhunushovA. T.

Жунушов Асанкадыр Темирбекович, д.в.н. профессор, член-корр. НАН КР,Директор института биотехнологии Национальной академии наук КР

г. Бишкек

Bishkek

zhunushov.asankadyr@gmail.comБердибаеваА. Б.BerdibaevaA. B.

Бердибаева Аида Бердибаевна, к.б.н., ученый секретарь института биотехнологии Национальной академии наук КР

г. Бишкек

Bishkek

aidaberdibaeva@rambler.ruИнститут биотехнологии Национальной академии наук КРКыргызстанInstitute of Biotechnology of the National Academy of Sciences of the Kyrgyz RepublicKyrgyzstan2023120920240161827Copyright © Жунушов А.Т., Бердибаева А.Б., 20242024Жунушов А.Т., Бердибаева А.Б.Zhunushov A.T., Berdibaeva A.B.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.biosafety.kz/jour/article/view/140

Появление новых вариантов коронавируса SARS-CoV-2 вызвано мутациями в основных структурных белках тяжелого острого респираторного синдрома коронавируса 2. Вакцинация и другие терапевтические подходы могут помочь остановить эпидемию. В настоящее время ученые разрабатывают препараты и вакцины, которые специально нацелены на структурные белки коронавируса SARS-CoV-2. В результате учет мутаций в белках и определение их влияния на функции помогут в высококачественном производстве и разработке профилактических и лечебных средств. В результате секвенирования удалось получить полную нуклеотидную последовательность Е гена коронавируса SARS-CoV-2. Наличие и местоположение мутаций белка оболочки (E) изолятов вируса SARS-CoV-2 были исследованы путем выравнивания последовательностей с референтной последовательностью вируса SARS-CoV-2. Результаты показали, что наиболее относительные мутации в аминокислотной последовательности белка E коронавируса SARS-CoV-2 произошли в регионах 9 и 11. Было обнаружено 2 мутаций Т9І и Т11А по сравнению со штаммом Severe acute respiratory syndrome coronavirus 2 (NC 045512.2). Выявленные структурные мутаций белка Е могут использоваться в стратегии разработки лекарственных препаратов и вакцин.

The emergence of new variants of the SARS-CoV-2 coronavirus is caused by mutations in the main structural proteins of severe acute respiratory syndrome coronavirus 2. Vaccination and other therapeutic approaches can help stop the epidemic. Scientists are currently developing drugs and vaccines that specifically target the structural proteins of the SARS-CoV-2 coronavirus. As a result, taking into account mutations in proteins and determining their impact on function will help in high-quality production and development of preventive and therapeutic agents. As a result of sequencing, it was possible to obtain the complete nucleotide sequence of the E gene of the SARS-CoV-2 coronavirus. The presence and location of mutations in the envelope protein (E) of SARS-CoV-2 virus isolates were investigated by aligning the sequences with the reference sequence of the SARS-CoV-2 virus. The results showed that the most relative mutations in the amino acid sequence of the E protein of the SARS-CoV-2 coronavirus occurred in regions 9 and 11. Two mutations, T9I and T11A, were found compared with the Severe acute respiratory syndrome coronavirus 2 (NC 045512.2) strain. The identified structural mutations of the E protein can be used in the strategy for developing drugs and vaccines.

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